Briefly, the synthesized DNA oligos for sgRNA-expressing plasmid construction were annealed and cloned into pGL3-U6-sgRNA-PGK-EGFP with U6 promoter (Addgene #107721). Oligos are showed in Supplementary Table 1. Base editors were constructed by insertion of amplified DNA product into linearized ABEmax (NdeI/Bg1II digest of pCMV_ABEmax, Addgene #112095). DNA products were amplified by Phanta Max Super-Fidelity DNA Polymerase (Vazyme, P505) using mutant site-containing primers (such as TadA-N46A-F/R) and two fragments primer sets (CMX-NdeI-F and Cas9n-Bg1II-R). Primers used are shown in Supplementary Table 2.

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