BM cells were isolated from the femur and tibia of B6 CD45.1/2+, B6 CD45.2+, and T-MHC I CD45.2+ donor mice. Following isolation, cells were counted and washed twice in cold phosphate-buffered saline (PBS). B6 CD45.1/2+ cells (5 × 105) mixed with 4.5 × 106 T-MHC I CD45.2+ cells were mixed and transferred intravenously into lethally irradiated (900 rads) CD45.1 recipient mice. The control group received 5 × 105 B6 CD45.1/2+ cells mixed with 4.5 × 106 B6 CD45.2+ cells. Mice were killed 8 weeks post reconstitution when the spleen and thymi were collected for flow cytometry analysis.

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