The Nlrc5-stopflox transgenic mice were generated by the insertion of a CAG-LoxP-STOP-LoxP-Nlrc5-WPRE-pA construct into mouse Rosa26 locus through the CRISPR/Cas9 system with C57BL/6J embryo. The generated mice were screened and further verified for the correct insertion by sequencing and Southern blotting. The generation of Nlrc5-stopflox mice was conducted by Biocytogen.

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