Total RNA was collected from E11.5 limb buds or lymphoblastoid cells using TRIzol (Thermo Fisher Scientific, catalog no. 15596026) and converted to cDNA using ReverTra Ace qPCR-RT master mix with genomic DNA (gDNA) remover (Toyobo, catalog no. FSQ-301). qPCR was performed using SsoFast EvaGreen supermix (Bio Rad, catalog no. 1725205). Primer sequences used for qPCR are shown in Supplementary Table 3.

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