PCR-Sanger sequencing (primers provided in Supplementary Table 3) was preformed using standard techniques28. For Southern blot analyses, genomic DNA were treated with BstXI (New England Biolabs, catalog no. R0113) and fractionated by agarose gel electrophoreses. Following capillary transfer onto nylon membranes, blots were hybridized with Digoxigenin (DIG)-labeled DNA probes (corresponding to chr5:29,348,565-29,349,037; mm10) amplified by the PCR DIG Probe Synthesis Kit (Sigma-Aldrich, catalog no. 11636090910). The hybridized probe was immunodetected with anti-digoxigenin Fab fragments conjugated to alkaline phosphatase (Sigma-Aldrich, catalog no. 11093274910) and visualized with a CDP star (Sigma-Aldrich, catalog no. 11685627001) according to the manufacturer’s protocol. Chemiluminescence was detected using the FluorChem E (ProteinSimple, catalog no.92-14860-00).

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