RNA-seq analysis was performed at Genminix Informatics Co., Ltd. (Shanghai, China). In brief, total RNA was extracted from SMMC-7721 cells with or without YAP knockdown. After quality analysis in an Agilent 4200 Bioanalyzer, samples were submitted for cDNA library construction. Then, the prepared libraries were sequenced on the Illumina HiSeq 2000 platform. Differential expression analysis was performed using the DESeq R package. Genes with an adjusted p value < 0.05 were considered differentially expressed.

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