Five-week-old nude mice were purchased from Bikai Laboratory Animal Corp. (Bikai, China). Bel-7402 cells treated as indicated were subcutaneously injected into mice at a density of 5 × 106. After xenografts were formed, piperazine erastin, which was purchased from MedChemExpress, was subcutaneously injected into the mice once a day. The tumors were measured 14 days after piperazine erastin injection, and tumor volumes were calculated with the formula 0.5 × L × W2, where L represents the length and W represents the width. The animal experiments were conducted under the institutional guidelines of Shanghai Children’s Medical Center.

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