Locomotion was recorded and analysed as described in Yatsuka et al.47. The trajectory plot data were recorded with a Visualix STD1 digital camera (Visualix, Kobe, Japan) attached to a Leica M80 microscope. The trajectory plot data were analysed using SMART video tracking software version 3.0.06 (PanLab, Harvard Apparatus, MA, USA; https://www.panlab.com/en/products/smart-video-tracking-software-panlab). Larvae at 6 dpf were placed into 12-well plates (one larva/well) in 1000 μl embryo medium (0.03% saltwater). The plate was placed under the Leica M80 microscope and tracked as follows: 30 min adaptation and 10 min tracking. All data of the locomotion analysis were recorded and analysed with the Zantiks MWP (Zantiks, Cambridge, UK). Larvae at 6 dpf were placed into 12-well plates (one larva/well) in 1000 μl embryo medium. The plate was transferred to the Zantiks MWP and tracked as follows: 30 min adaptation and 10 min tracking.

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