Iron detection was conducted using an Iron Assay Kit (Abcam) according to the manufacturer’s protocol to assess the total iron concentration. Generally, cells treated as indicated were harvested and lysed using iron assay buffer. An iron reducer was added to the supernatant and incubated at room temperature for 30 min in the dark. Subsequently, the iron probe was added, and the mixture was incubated for 1 h at room temperature. The absorbance at 593 nm was measured immediately using a microplate reader.

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