MDA concentrations were measured with a lipid peroxidation assay kit (Abcam, #ab118970) according to the manufacturer’s protocol. Briefly, cells treated as indicated were lysed using lysis buffer, and the supernatant was collected. Thiobarbituric acid (TBA) solution was added to the samples, and the mixture was incubated at 95 °C for 1 h. Then, the mixture was cooled to room temperature and added to a 96-well microplate. The absorbance at 532 nm was measured immediately using a microplate reader (Biotek).

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