Blood samples from the proband and parents were spun over Ficoll-Paque (Amersham Biosciences) gradients to enrich the sample for mononuclear cells. Epstein Bar virus (EBV)-transformed lymphoblastoid lines were generated from isolated peripheral blood lymphocytes. Briefly, cells were washed and resuspended in complete Iscove’s modified Dulbecco’s culture media supplemented with 10% v/v fetal bovine serum, antibiotics, and virus. The B95-8 EBV-infected marmoset cell line (ATCC, catalog no. CRL-1612) was used as the source for viral stocks. High molecular weight DNA was isolated from Ficoll-Paque enriched mononuclear cells using standard desalting procedures. Lymphoblastoid cells were maintained in RPMI1640 medium (Life Technologies, catalog no. 11875093) containing 15% fetal bovine serum (FBS) and penicillin-streptomycin.

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