Cell proliferation and colony formation assays were performed as described previously. Briefly, for the cell proliferation assay, cells treated as indicated were seeded into a 96-well plate at a density of 3000 cells per well. Then, the cells were incubated with CCK-8 reagent (Beyotime, China) for 1 h. The absorbance was measured at 450 nm. For the colony formation assay, cells were seeded into six-well plates at a density of 5000 cells per well in DMEM containing 0.3% agarose. After incubation for 2 weeks, colonies were counted.

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