We used whole extracts from whole tDRGs collected from adult WT mice for Western blotting48. Whole tDRG extracts were homogenized in ice-cold CelLytic MT Cell Lysis Reagent (Sigma-Aldrich) containing a protease and phosphatase inhibitor cocktail. We used 4 replicas per condition (PBS-control and LPS), and each replica was generated from 2 adult mice. Equal amounts of protein were loaded per group, separated on 12% SDS polyacrylamide gels and then electrotransferred onto a PVDF or nitrocellulose membrane (Bio-Rad Laboratories, Hercules, CA, USA). Membranes were incubated overnight at 4 °C with the following primary antibodies: rabbit anti-RAGE (1:1000; Abcam) and mouse anti-β-actin (1:2000; Sigma); followed by horseradish peroxidase-conjugated goat anti-rabbit or goat anti-mouse secondary antibodies (1:20,000; Bio-Rad Laboratories). Protein signals were visualized using enhanced chemiluminescence reagents (Bio-Rad) and quantified by densitometry using ImageJ software (NIH, Bethesda, MD, USA).

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