The liver cancer cell lines Bel-7402 and SMMC-7721 were obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). The HEK293T cell line was obtained from the American Type Culture Collection (Manassas, VA, USA). Cells were cultured in Dulbecco’s-modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum and maintained at 37 °C in 5% CO2. For induction of ferroptosis and O-GlcNAcylation, cells were treated with PUGNAc (25 µM, Sigma, St. Louis, MO, USA) and RSL3 (2 µM, Sigma), respectively. For inhibition of ferroptosis, apoptosis, and necrosis, cells were treated with ferrostatin-1 (2 µM, Sigma), ZVAD-FMK (6 µM, Selleck), and necrosulfonamide (1 µM, Selleck), respectively. The YAP-WT, YAP-sh, OGT-OE, and OGT-sh plasmids were obtained in our previous studies19. The TFRC-sh, TFRC-OE, SLC7A11-sh, and SLC7A11-OE plasmids were purchased from GeneChem (Shanghai, China). The plasmids with truncated TFRC promoter regions that were cloned into pGL3 were purchased from Sangon Biotech (Shanghai, China).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.