The primary antibodies used in this study were polyclonal rabbit anti-Vasa (1:250, sc-30210; Santa Cruz), mouse anti-PAR (1:20, ab14459; Abcam), rabbit anti-cleaved Dcp-1 (1:100, 9578 S; Cell Signaling), rabbit anti-Hb9 (1:100; from J. Skeath, Haverford College, PA), and the following antibodies from the Hybridoma Bank (DSHB): polyclonal rabbit anti-Arl8 (1:20) and monoclonal mouse anti-Vasa (direct, 46F11) and mouse anti-γH2Av (direct, UNC93-5.2.1).

Anti-DNase II antibodies were generated as follows: GST-tagged DNase II protein without the signal peptide (spanning amino acids 1-19) was expressed in E. coli. A Coomassie Blue-stained gel slice of the corresponding size was used as an antigen to generate polyclonal antibody in two rats. One immunization and four boosts were done for each animal and after the 4th boost 60 ml serum were obtained. The antibody was used at a dilution of 1:20.

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