The antibody was labeled with Alexa Fluor 488 and 647 using an Antibody Labeling Kit (Invitrogen, A20181 and A20173). The operation was basically performed according to the attached document. Briefly, the antibody was incubated with an Alexa Fluor dye and the resulting reaction solution was purified by a Zeba Spin Column (Thermo Scientific, 89883) to remove the free dye. The concentration of the antibody and labeling efficiency was determined by a NanoDrop Spectrophotometer (Thermo Scientific, United States).

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