Adenoviruses were prepared using the ViraPower Adenoviral Expression System (Invitrogen), as previously described1,19, 20,21. PCR-amplified, C-terminal 6xHis and 3xFLAG tagged full-length Ildr2 was subcloned into the pENTR/D-TOPO vector using the pENTR Directional TOPO Cloning Kit (Invitrogen). Inserts of pENTR-Ildr2-6xHis-3xFLAG vector were transferred into the pAd/CMV/V5-DEST vector by the Gateway system (Invitrogen).

For the shRNAs of Ildr2 and LacZ were cloned into BLOCK-iT U6 entry vector (Invitrogen). The sequence of the shRNA for Ildr2 shRNA1 was: 5′- cacc GAAGAAGGTGGCCATGCTC acgtgtgctgtccgt GAGCATGGCCACCTTCTTC -3′ and Ildr2 shRNA2 was: cacc GCTGATTTCAAATCTTAGT gcgcttcctgtcacgc ACTAAGATTTGAAATCAGC. The inserts of pENTR shRNAs vectors were transferred into the adenovirus vector pAd/PL-DEST using the Gateway system (Invitrogen). The recombinant adenoviruses were purified by the Adenovirus Purification Miniprep Kit (Cell Biolabs).

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