Sodium dodecyl sulfate (SDS)-page gel electrophoresis
This protocol is extracted from research article:
The molecular pH-response mechanism of the plant light-stress sensor PsbS
Nat Commun, Apr 16, 2021; DOI: 10.1038/s41467-021-22530-4

SDS-page gel electrophoresis analysis (12.5% running gel, 4% stacking gel stained with Coomassie brilliant blue R-250 Bio-Rad) was carried out for checking the yield of PsbS at every step of overexpression, purification, and refolding. For staining, 2.5 µL of Precision Plus Protein™ Dual Color Standard from Sigma was used.

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