Genomic DNA was extracted from fresh mycelia growing on PDA after 3–5 days of growth following the CTAB method described in Watanabe et al. (2010). For amplification of the ITS, TEF1, GAPDH and RPB2 gene fragments, the primer pairs ITS5/ITS4 (White et al. 1990), EF1-728F/EF1-986R (Carbone and Kohn 1999), gpd1/gpd2 (Berbee et al. 1999) and RPB2-5F/RPB2-7cR (Liu et al. 1999) were used, respectively. A total of 25 μL of a PCR reaction mixture containing 21μL of 1.1×Taq PCR Star Mix (TSINGKE, Beijing, China), 2 μL template DNA and 1μL of each primer was prepared and the PCR was performed in an Eppendorf Mastercycler following the protocols described by Woudenberg et al. (2013). Successful products were purified and sequenced by TSINGKE company (Beijing, China). All sequences were assembled with BioEdit (Hall 1999) and deposited at GenBank ( (Table (Table11).

Alternaria strains and their accession numbers used in the phylogenetic analysis.

Notes: Alternaria strains of the present study are marked in bold. Type strains are marked ‘T’. Representative strains are marked ‘R’.

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