The AAR results were saved in pdf file format and digitized with WebPlotDigitizer 4.2 software (Automeris; San Francisco, California, USA). Pixel-based image data were converted to numerical xy coordinates. The raw data were thresholded with RGB color for congested right (255, 223, 204) ± 10, congested left (103, 148, 198) ± 10, decongested right (232, 138, 135) ± 10 and decongested left (193, 218, 242) ± 10 to separate the AAR curves. A discretization window of 5 × 5 pixels was chosen; the picture resolution was 847 × 757 pixels. More than 1500 xy points per AAR curve were saved in csv file format.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.