Mouse embryonic fibroblasts (MEFs) were obtained from E15.5 HIPKI and HIPKI-C331G mouse embryos by clipping the tail tips and culturing in media (DMEM (with glucose) supplemented with 10% fetal calf serum, 100 U/ml penicillin, 100 U/ml streptomycin at 37°C in a humidified chamber with 5% CO2). HIPKI and HIPKI-C331G MEFs, HEK293T, MCF7 and SW480 cells were maintained in DMEM (Lonza) and supplemented with 10% fetal calf serum, 100 U/ml penicillin, 100 U/ml streptomycin at 37°C in a humidified chamber with 5% CO2.

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