Human pancreatic islets were obtained through the European Consortium on Islet Transplantation (ECIT), Islets for Basic Research Program supported by the Juvenile Diabetes Research Foundation (program 2-RSC-2019-724-I-X). Pancreatic islets were isolated from multiorgan donors without a history of glucose intolerance (Nano et al., 2015), shipped in culture medium and re-cultured at 37°C in a humidified chamber with 5% CO2 in glucose-free RPMI 1640 supplemented with 10% fetal calf serum, 100 U/ml penicillin, 100 U/ml streptomycin and 11mM glucose for three days before analysis.

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