P. berghei parasites engineered to express P. falciparum CSP in place of the endogenous P. berghei CSP molecule (Pb-PfSPZ) (Espinosa et al., 2017) were used throughout the study. Parasites were maintained by serial passage through Anopheles stephensi mosquitoes.

Mice were immunized IV with 5 × 104 irradiated (15kRad) Pb-PfSPZ dissected by hand from the salivary glands of Anopheles stephensi mosquitoes.

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