C57BL/6 mice, MD4 (Goodnow et al., 1989), B1-8 mice (Shih et al., 2002b) and Ighg2A10 (McNamara et al., 2020) were bred in-house at the Australian National University. All mice were on a C57BL/6 background. Mice used for the experiment were 5 to 8 weeks, and they were age matched for each experiment groups. Mostly female mice were used throughout the experiments, and were bred and maintained under specific pathogen free conditions in individually ventilated cages at the Australian National University.

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