Live cell time-lapse imaging
This protocol is extracted from research article:
Long-term live cell cycle imaging of single Cyanidioschyzon merolae cells
Protoplasma, Jan 5, 2021; DOI: 10.1007/s00709-020-01592-z

Live cell time-lapse images were captured using a confocal fluorescence microscope (OLYMPUS FV3000). As the objective lens, we used USLSAPO 100XS (OLYMPUS) with silicon oil, because regular oil ruptures the bottom membrane of the ibidi dish. β-tubulin-sfGFP T1 cells were seeded on the ibidi dish and incubated at 42 °C, 5% CO2. We set a white LED light on the stage top incubator. The light intensity was set at 15 μmol/m2/s. Time-lapse imaging was started 4 h after the cell seeding. The time-lapse images were obtained every 15 min for 40 h.

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