The wild-type C. merolae strain, MO, and the uracil-auxotrophic mutant, T1, were used in this study. In T1, the URA5.3 gene is completely deleted, which allows a backgroundless selection of transformants (Taki et al. 2015). The strains were cultured in gyratory culture (120 rpm) at 42 °C under continuous light (50 μmol/m2/s) in MA2 medium (pH 2.5) and MA2 medium containing uracil (0.5 mg/ml), respectively (Ohnuma et al. 2008).

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