RNA quantification and qualification of RNA degradation and contamination were performed on 1% agarose gels. The purity of RNA was checked using a NanoPhotometer® spectrophotometer (IMPLEN, CA, USA), and the integrity of RNA was assessed using the RNA Nano 6000 Assay Kit of the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA).

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