The wild-type AB strain of zebrafishes (Danio rerio) were obtained from Hunter Biotechnology (Hangzhou, China). The zebrafishes were maintained in the 28°C reverse osmosis water, incubating with 200 mg/L instant sea salt and 50–100 mg/L CaCO3, at an electrical conductivity of 450~550 μS/cm and a pH of 6.5–8.5. The embryos used in this study were generated by natural pairwise mating.

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