Human neuroblastoma (SH-SY5Y) cells were provided by China Infrastructure of Cell Line Resource. SH-SY5Y cells were cultured in Dulbecco's modified Eagle medium (DMEM) (Corning), supplemented with 10% fetal bovine serum (FBS) (Corning) and 1% penicillin-streptomycin (Beyotime Biotechnology Inc., Nantong, China). The cells were incubated at 37°C under a humidified atmosphere of 5% carbon dioxide.

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