2.11. Reverse Transcription-Polymerase Chain Reaction

Total RNA was extracted using TRIzol reagent. After quantifying RNA, a reverse transcription kit was used to synthesize cDNA to generate a 20 µl reaction system. The primer sequence used was as follows. Bcl-2 forward: 5′-TGGACAACCATGACCTTGGAC-3′, reverse: 5′-GTGCTCAGCTTGGTATATGAGAA-3′; caspase8 forward: 5-′ATGAGCTGGGCTGAAGCAAAC-3′, reverse: 5′-AAGACCTCAATTCTGATCTGCTCAC-3′; caspase9 forward: AAGCCAACCCTAGAAAACCTTACC-3′, reverse: 5′-GACATCACCAAATCCTCCAGAAC-3′; BAX forward: 5′-CGACTGATGTCCCTGTCTCCA-3′, reverse: 5′-AGCACTCCCGCCACAAA-3′; caspase3 forward: 5′-GACTCTGGAATATAAATGGACAACA-3′, reverse: 5′-AGGTTTGCTGATCGACATCTG-3′, ACTB forward: 5′-TGGCACCCAGCACAATGAA-3, reverse: 5′-CTAAGTCATAGTCCGCCTAGAAGCA-3′; ACTB was used as an internal reference gene.

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