2.4. High Content Analysis (Perkin Elmer) System for Real-Time Monitoring of Cell Proliferation

A seeding density of 2,000 cells per well was grown in 96-well culture plates. The cells were divided into control (0.1% DMSO) and alloimperatorin (25, 50, 100, 150, and 200 μM) treated groups. A PerkinElmer Operetta CLS high-content imaging analysis system was used for live cell dynamic monitoring. The cells were cultured at 37°C (5% CO2). A select digital phase contrast channel, a 10× objective lens, and full-hole shooting were used to monitor cell proliferation. The shooting was performed once every 1 h, for 66 h. Harmony software was used to measure dynamic cell proliferation for 66 h.

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