To confirm the role of RA on Nrf2-mediated protection, Nrf2-KO endothelial cells were generated using the CRISPR/Cas9 gene-editing tool. EA.hy926 cells were transfected with Nrf2-CRISPR/Cas9-GFP plasmid (sc-400017, Santa Cruz, USA). GFP+ cell expression was screened using FACS. Western blot analysis for Nrf2-expression was performed to confirm successful Nrf2-depletion. Further, the Nrf2 role in RA which facilitated the protection of endothelial cells against ER stress was assessed using Nrf2 KO cells by assessing the expression of ER stress markers and cell viability.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.