The LG of rabbits was immediately collected after euthanasia. After rinsing with normal saline, the LG was fully homogenized in an ice bath, diluted with 300 μ saline, and centrifuged. The supernatant was collected and stored at −80°C for further experiments. ACh and α7nAChR were detected using the ELISA kit, and their contents were determined using a microplate reader.

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