2.3. Network Construction of Compound-Target-Pathway in SCL for PID Treatment

Systems pharmacology was applied to analyze the interaction between SCL and PID and the selection of critical targets. To identify the intersection of targets between PID and SCL, the title of “pelvic inflammatory disease” was placed in GeneCards (https://www.genecards.org/) [40], DisGeNET (https://www.disgenet.org/) [41], DrugBank (https://www.drugbank.ca/) [42], and existing research [43,44] to obtain gene names of PID targets, which manually confirmed that each target had a clinical study in PID. Additionally, the intersection genes (compound- and disease-related targets) were used to perform annotation analysis of the obtained crossover genes by using Gene Ontology (GO) and the KEGG pathway analysis functions in the STRING platform (https://string-db.org/) [45], with the intersection genes directly mapped to the pathway. Cytoscape [46] was used to visualize a network of “Compound-target-pathway (C-T-P network)”. In this network, each compound, target, and pathway was indicated by nodes, and the interactions between each node were described by edges. The network was established to project an overview of the interactions among compounds, targets, and pathways.

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