The corneal staining filter paper strip was placed into the lower eyelid fornix of the rabbit and wetted, and the fluorescein was rapidly and evenly distributed on the cornea though eye blink. The corneal epithelial injury was graded with the cobalt blue filter. The cornea was classified into four quadrants, and the score was determined and shown as follows: absent, 0; less than five spots, 1; more than five spots, 2; and large-area fluorescein plaque, 3. Finally, the score of each grade was added, and the full score was 12.

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