25 mg liver tissue was homogenized with 250 μl RIPA buffer and then centrifuged at 1600 ×g and 4°C for 10 minutes. The supernatant was collected and stored at −80°C for use. The superoxide dismutase (SOD) activity in the liver was analyzed by Randox assay kit (Cat. no. SD125, Randox Laboratories, ANT, UK) with absorbance at 340 nm. The catalase assay kit (Cat. no. 707002, Cayman Chemical, MI, USA) was used with 540 nm adsorption rate for the detection of catalase activity in the liver. For analyzing the levels of thiobarbituric acid reactive substances (TBARS) in mouse liver, 100 μl homogenized liver supernatant was mixed with 100 μl sodium dodecyl sulfate (SDS) solution and 4 ml color reagent under boiled water for an hour and then ice-cooled. The mixture was centrifuged at 1600 ×g under 4°C for 10 minutes and then the absorbance value was read at 535 nm spectrophotometrically. The data were expressed as equivalent malondialdehyde (MDA) µM/g protein [26].

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