On the fourth week of the experiment, the SAMP8 mice blood was collected from the orbital sinus and centrifuged at 6000 rpm and at 4°C for 5 minutes and then stored at −20°C. The following parameters were analyzed by Beckman D × C 800 chemistry analyzer (Beckman Coulter, CA, USA): glucose, total protein, albumin, triglycerides, total cholesterol, high-density/low-density lipoprotein cholesterol (HDL-C/LDL-C), glutamate oxaloacetate transaminase (GOT), glutamic pyruvic transaminase (GPT), blood urea nitrogen (BUN), and creatinine.

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