MC3T3-E1 cells were purchased from the Institute of Life Science Cell Culture Center (Shanghai, China), rBMSCs were purchased from Cyagen Biosciences Inc. (CA, USA), PDLPs were purchased from the Institute of ScienCell Research Laboratories (Carlsbad, CA, USA). The cells were cultured in growth medium (GM), containing α-MEM medium (Gibco, USA) supplemented with 1% penicillin-streptomycin solution (P/S, Gibco) and 10% fetal bovine serum (FBS, Gibco, USA) at 37 °C in 5% CO2. For osteogenic induction, cells were cultured in an medium which containing GM supplemented with 0.1 μM dexamethasone, 50 μg/mL ascorbic acid and 10 mM β-glycerophosphate.

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