MicroComputerized Tomography (µCT) was used for visualization, evaluation of 3D dispersion and retention of NBTXR3 within the tumor. For µCT acquisition, mice were anesthetized using isoflurane (delivered as 1–3% for maintenance; up to 5% for induction) in oxygen from a precision vaporizer. Micro-CAT II CT scanner (Siemens) in which X-rays were generated at 80 kVp and 500 mA was used as imaging acquiring equipment (Voxcan).

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