For the in vivo tumor growth control assays, cells were subcutaneously injected into one flank of mice. Once the tumor had grown (50–120 mm3), mice were randomized to the different groups. A volume of NBTXR3 suspension (or vehicle) corresponding to 25% of the baseline tumor volume was injected into the tumor. After 24 hours, tumors were irradiated. The non-tumor parts of the mice were shielded by lead blocks. For chemoradiotherapy experiments with NCI-H460 cells, CDDP (1mg.kg−1) was administered by intraperitoneal injection two hours prior to RT, daily over 5 consecutive days. Length (L) and width (W) of tumors were measured 2–3 times per week using a digital caliper. Tumor volumes were calculated using the formula (L×W2/2).

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