The macrophages were treated with 20 ng/mL IL-4 and 20 ng/mL IL-13 (for M2 polarization), or HepG2 cell-derived exosomes for 24 h. The macrophages surface makers CD86 (M1 marker) and CD206 (M2 marker) were analyzed by flow cytometry (BD Biosciences).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.