For clonogenic assays, cells were seeded in triplicate into 6 well plates in a range of 200–10,000 cells/well depending on the test condition and radiation dose. Once cells were attached to the plate, 50µM to 800µM NBTXR3 was added. Different doses of X-rays were delivered 16 hours post NBTXR3 treatment. The cells were cultured up to 15±2 days at 37°C under 5% CO2. The colonies were fixed and stained with crystal violet (Sigma) (25% in EtOH) 2 mL/well. All colonies of 50 cells or more were then counted. For clonogenic assay with NCI-H460-Luc2 cells, the same protocol was followed except that cells were exposed to 725nM of CDDP for 6 hours or 405nM of CDDP for 16 hours. Then, the medium was replaced by fresh medium without CDDP before irradiation.

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