For in vitro experiments, cells were irradiated at various doses according to the study, delivered as a single dose, using a 200kV X-ray source (NDI 226, Varian). For in vivo experiments, subcutaneous tumors were irradiated with various doses, using the same device as for in vitro experiments, excepted for PC-3 tumors irradiated using a 150kV X-ray source (CellRad, Faxitron), and NCI-H460 tumors irradiated using a 310kV X-ray source (Therapax, Agfa NDT). Selective irradiation of the tumor on mice was performed by the interposition of a lead shield, allowing full protection of the rest of the body, including proximal lymph nodes.
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