Biotinylated hsa_circ_0074854 was obtained from GenePharma (Shanghai, China). HepG2 cells were transfected with biotinylated hsa_circ_0074854 for 24 h. After that, cell lysates were cultured with streptavidin-coated magnetic beads (Thermo Fisher Scientific). The biotin-coupled RNA complex was pulled down and the level of HuR was detected by RT-qPCR.

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