2.6. Effect of Diannexin on the Oxidative Stress Response

The lung tissue was homogenized with 0.9% saline (1 : 9 weight ratio) and then centrifuged at 1,000 × g for 15 min at 4°C to obtain the supernatant. We measured malondialdehyde (MDA) concentrations and myeloperoxidase (MPO) activity using respective kits (Nanjing Jiancheng, Nanjing, China). The expression of iNOS was also detected using western blot analysis.

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