2.4. Effect of Diannexin on Alveolar-Capillary Permeability

Alveolar-capillary permeability was estimated using the oxygen index, wet/dry weight ratio, and BALF protein levels. PaO2 was analyzed using the RapidLab 348 system (Bayer Diagnostics, Germany), and the oxygen index was calculated using the PaO2/FiO2 ratio. Part of the right upper lobe was weighed and dried at 60°C in a drying oven for 48 h and then weighed again to calculate the wet/dry weight ratio. BALF protein concentrations were measured using a BCA kit (Thermo Fisher, Shanghai, China).

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