2.3. Differential expression genes (DEGs) between HCC primary tumors (HCC) and HCC tumor adjacent tissues (HTA)

The DEGs between HCC (n = 255) and HTA (n = 50) tissues were calculated. In addition, we divided primary tumors into VIR (n = 97) and NVIR (n = 158) according to clinical information for further study. For assay, the deregulation gene expression, “Limma” package of R software was employed to test the DEGs. The mRNAs with log2 fold change |log FC| >1 (FDR adjusted P-value < .01) were considered to be significantly differentially expressed mRNAs.

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