RUT (urea agar-based medium), histopathology (Giemsa, and hematoxylin and eosin stains), and multiplex PCR for H. pylori were performed on the gastric biopsies. H. pylori was also genotyped by a validated multiplex PCR assay directly from the gastric biopsy (17). H. pylori was considered positive if either PCR or both RUT and histology were positive.

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