All bright field and fluorescent images were recorded using an AxioZoom.V16 microscope (Zeiss), equipped with a movable stage. Phase-contrast images of cuticle preparations were recorded using an Axioplan2 (Zeiss).

For live imaging, eggs were carefully removed from the floral foam and washed with tap water. Subsequently, eggs were placed on 1.5% agarose and covered with Voltalef H10S oil. Live imaging was performed at 25–27°C.

Projections of image stacks were carried out using Helicon Focus (HeliconSoft). Movies were created by using Fiji (Schindelin et al., 2012). Images have been adjusted for brightness and contrast using Adobe Photoshop CS5.1.

False-colour overlays of in situ hybridisation images were generated as described in Pechmann et al., 2017.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.