2.3.3. Linear range verification
This protocol is extracted from research article:
Performance evaluation of CA242 by flow fluorescence assay
Medicine (Baltimore), Apr 16, 2021; DOI: 10.1097/MD.0000000000025301

Following the EP6-A guideline published by the CLSI,[12] the concentrations of L-Series standard solutions (L1-L5, known concentrations of 15.84 U/ml, 120.34 U/ml, 224.83 U/ml, 329.33 U/ml and 433.82 U/ml, respectively) were detected by the testing kit. All raw fluorescence intensity data were converted into corresponding concentrations through the calibration curve and a scatter diagram was made (X: theoretical concentration, Y: instrumental concentration). Then, multiple regression models were applied to analyze the linear range.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.